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Amperometric biosensor based on diamond paste for the enantioanalysis ofl-lysine

Authors
Journal
Biosensors and Bioelectronics
0956-5663
Publisher
Elsevier
Publication Date
Volume
35
Issue
1
Identifiers
DOI: 10.1016/j.bios.2012.02.036
Keywords
  • L-Lysine
  • Amperometric Biosensor
  • Monocrystalline Diamond
  • Amino Acid
Disciplines
  • Pharmacology

Abstract

Abstract An amperometric biosensor was proposed for the enantioanalysis of l-lysine. The biosensor is based on the impregnation of l-lysine oxidase in diamond paste. The potential used for the determination of l-lysine was 650mV. The biosensor exhibited a linear concentration range between 1 and 100nmol/L with a limit of detection of 4pmol/L. The selectivity of the biosensor is high over other amino acids, such as l-serine, l-leucine, l-aspartic acid, l-glutamic acid, histamine, glycine. The proposed biosensor can be applied for the determination of l-lysine in serum samples and pharmaceutical compounds.

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