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Association of C807T, PlA, and −5 C/T Kozak genotypes with density of glycoprotein receptors on platelet surface

Authors
Journal
Thrombosis Research
0049-3848
Publisher
Elsevier
Publication Date
Volume
112
Issue
3
Identifiers
DOI: 10.1016/j.thromres.2003.12.006
Keywords
  • Platelets
  • Glycoprotein
  • Polymorphism
Disciplines
  • Biology

Abstract

Abstract This study investigates whether three platelet glycoprotein (GP) polymorphisms, C807T in GP Ia, Pl A1/A2 in GP IIIa, and −5 T/C Kozak in GP Ibα gene, influence the density of the three important adhesion and activation receptors on the platelet surface. Fifty-four healthy donors were genotyped according to the three polymorphisms, and densities of the corresponding GPs were measured by flow cytometry. Our study confirmed the association between C807T polymorphism and platelet surface expression of GP Ia–IIa and GP Ia and demonstrated that the density of GP Ibα or GP IX is not associated with the Kozak polymorphism. Although the Pl A1/A2 polymorphism did not affect the expression of GP IIb–IIIa and GP IIIa on the platelet surface, flow-cytometric analysis employing murine monoclonal antibody SZ21 against GP IIIa can be applied to distinguish Pl A1/A1 and Pl A1/A2 polymorphism.

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