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HTLV-1 affects the adipogenic differentiation of human mesenchymal stem cells

Authors
Journal
Retrovirology
1742-4690
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Volume
8
Identifiers
DOI: 10.1186/1742-4690-8-s1-a115
Keywords
  • Meeting Abstract
Disciplines
  • Biology

Abstract

HTLV-1 affects the adipogenic differentiation of human mesenchymal stem cells MEETING ABSTRACT Open Access HTLV-1 affects the adipogenic differentiation of human mesenchymal stem cells Evandra Strazza Rodrigues1,2*, Maurício C Rocha Jr1,2, Maristela Delgado Orellana1,3, Osvaldo Massaiti Takayanagui3, Dimas Tadeu Covas1,3, Simone Kashima1,2 From 15th International Conference on Human Retroviruses: HTLV and Related Viruses Leuven and Gembloux, Belgium. 5-8 June 2011 Background Mesenchymal stem cells (MSCs) are multipotent cells mainly found in the bone marrow (BM) and are consid- ered to support human hematopoiesis. During HTLV-1 infection, T cells that are infected may migrate to the BM, and establish a progressive viral invasion. The per- sistence of these cells in the BM may affect the function of the resident BM cells and the biological properties of this microenvironment. However, the exact mechanisms of this phenomenon and the relative contribution of HTLV-1 to BM cell dysfunction remain to be eluci- dated. In this study, we evaluated the ability of HTLV-1 to disturb MSCs normal functions. Methods Human MSCs were cultured on the presence of MT-2 cell line. MSCs markers and adipogenic differentiation potential was evaluated. Results We observed that MSCs that had contact with HTLV-1 were able to differentiate into adipocytes. However, gene expression of PPARG, cell morphology and intracellular lipid formation analyses revealed that adipogenic differ- entiation of MSCs that were exposed to HTLV-1 was decreased compared to MSCs not exposed (p<0.03). In addition, analyses by flow cytometry showed a reduction of the expression of MSCs markers (CD271 and HLA- DR) in MSCs that had contact with HTLV-1. Conclusion HTLV-1 caused significant changes in the characteristics and functions of MSCs. This finding suggests the importance of HTLV infection on MSCs, and this could contribute to the understanding the viral infection mechanisms and pathogenesis. Acknowledgements Financia

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