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A sensitive automated colorimetric method for the determination of serum gamma-glutamyl transpeptidase

Authors
Journal
Clinica Chimica Acta
0009-8981
Publisher
Elsevier
Publication Date
Volume
69
Issue
1
Identifiers
DOI: 10.1016/0009-8981(76)90469-1

Abstract

Abstract A highly sensitive and accurate automated method using a Technicon Auto-analyzer AAII was developed for the determination of human serum γ-glutamyl transpeptidase. γ-L-Glutamyl- p-nitroanilide added as substrate was split to p-nitroaniline and the γ-glutamyl group was trapped by glycylglycine in Ammediol/HCl buffer solution. Liberated p-nitroaniline was diazotized and converted by Tsuda Reagent ( N, N-(diethyl)- N′-(l-naphthy1)ethylendiamine) to an azo-dye possessing an absorbance maximum at 550 nm. This new method of determination, using the conversion of p-nitroaniline to azo-dye, was found to be 7 times more sensitive as compared to the conventional direct p-nitroaniline determination method [1]. This method requires only 0.1 ml serum and permits 60 determinations per hour.

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