Affordable Access

Visualization of Z sequences in form V of pBR322 by immuno-electron microscopy.

Authors
Publication Date
Source
PMC
Keywords
  • Research Article

Abstract

Form V DNA has been prepared from pBR322 DNA by annealing covalently closed complementary single strands. Specific rabbit antibodies to Z-DNA were shown by radioimmunoassay and electron microscopy to react with form V DNA of pBR322. The bound antibodies were visualized either directly (on synthetic polynucleotides in Z-form), or after reaction with goat anti-rabbit immunoglobulin labeled with ferritin (on form V DNA).

There are no comments yet on this publication. Be the first to share your thoughts.