Affordable Access

Publisher Website

17β-Hydroxysteroid oxidoreductase: A ubiquitous enzyme. Interconversion of estrone and estradiol-17β inBALB cmouse tissues

Authors
Journal
Metabolism
0026-0495
Publisher
Elsevier
Publication Date
Volume
34
Issue
10
Identifiers
DOI: 10.1016/0026-0495(85)90142-8
Disciplines
  • Biology

Abstract

Abstract A survey was conducted to define the sites of 17β-hydroxysteroid oxidoreductase activity in organs and tissues of male and female BALB c mice, as well as the favored direction of the oxidoreductase reaction in intact tissues. The enzyme activity was assayed by use of radiolabeled estrone and estradiol-17β as substrates. Estrone formation from estradiol-17β was demonstrated in all tissues. The formation of estradiol-17β from estrone was demonstrated in most tissues, however, it was barely detected or was undetectable in the glandular stomach, small intestine, cecum, and large intestine. Thus, 17β-hydroxysteroid oxidoreductase activity is expressed in all BALB c mouse organs and tissues. Approximately two-thirds of the tissues and organs examined, including those of the reproductive tracts, favored the conversion of estrone to estradiol-17β rather than the reverse reaction. The results of this study, however, represent qualitative estimates of 17β-hydroxysteroid oxidoreductase activity in BALB c mouse tissues that are uncorrected for conversion to hydroxylated metabolites. These in vitro findings suggest that the 17β-hydroxysteroid oxidoreductase catalyzed reduction of estrone may contribute to the maintenance of physiologic levels of estradiol-17β in estrogen responsive tissues.

There are no comments yet on this publication. Be the first to share your thoughts.