Affordable Access

Publisher Website

Fractionation of ribonucleic acids with phenol:IV. Chromatographic analysis of a rapidly labelled RNA fraction of the normal rat liver

Authors
Journal
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis
0005-2787
Publisher
Elsevier
Publication Date
Volume
108
Issue
4
Identifiers
DOI: 10.1016/0005-2787(65)90051-1

Abstract

Abstract A RNA fraction characterized by a high rate of synthesis has been separated from the bulk of RNA of normal rat liver by a technique of RNA fractionation with phenol. This fraction was concentrated in the phenol-water interphase and extracted from it in an “undegraded” form by using bentonite throughout the procedure. Chromatography of this RNA on a methylated bovine serum albumin column showed that its main component resembled ribosomal RNA and that a rapidly labelled RNA component was eluted behind the main component. Further chromatography of the radioactive peak gave fractions differing considerably in the rate of isotopic labelling but with guanine plus cytosine contents consistently lower than that of the bulk of the cellular RNA. A fraction with the highest rate of labelling had the lowest guanine plus cytosine content.

There are no comments yet on this publication. Be the first to share your thoughts.