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Dissimilation of citric acid by extracts ofPenicillium chrysogenum

Authors
Journal
Archives of Biochemistry and Biophysics
0003-9861
Publisher
Elsevier
Publication Date
Volume
86
Issue
1
Identifiers
DOI: 10.1016/0003-9861(60)90376-3
Disciplines
  • Biology

Abstract

Abstract Cell-free extracts prepared from mycelium of Penicillium chrysogenum Q176 oxidized citrate, cis-aconitate, and isocitrate. In addition, citrate was formed from oxalacetic acid and a 2-carbon fragment in the presence of fluoroacetate. When citrate was metabolized aerobically by these extracts, one-half mole of oxygen uptake was observed for every mole of α-ketoglutarate formed. Carbon dioxide evolution was equivalent to the citrate dissimilated and, in crude extracts, far in excess of the keto acids formed. Under anaerobic conditions, citrate could be dissimilated quantitatively to glutamate and carbon dioxide by a TPN-mediated pathway, and the amount of citrate dissimilated was dependent on the ammonium-ion concentration. This pathway explains the “excess” carbon dioxide formed when citrate is dissimilated aerobically. The presence of isocitritase activity in these extracts was confirmed. This enzyme has a pH optimum of 6.0–6.2 and a bivalent metal-ion requirement satisfied by either magnesium or manganese.

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