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In vivo radioactive labeling ofArbaciasperm histones

Authors
Journal
Experimental Cell Research
0014-4827
Publisher
Elsevier
Publication Date
Volume
71
Issue
2
Identifiers
DOI: 10.1016/0014-4827(72)90321-7
Disciplines
  • Biology

Abstract

Abstract Sperm histones of Arbacia were labeled in vivo by a single intracoelomic injection of 3H-arginine 10 days prior to respawning. Histones were fractionated on polyacrylamide gels and counted. Radioactivity was found in all histone fractions in amounts approximately proportional to the amount of protein in each band. This technique provides a way of obtaining histone-labeled sperm which could be used to trace the fate of sperm nucleoproteins after fertilization.

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