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Organization of telomeric and sub-telomeric regions of chromosomes from the protozoan parasiteTrypanosoma cruzi

Authors
Journal
Molecular and Biochemical Parasitology
0166-6851
Publisher
Elsevier
Publication Date
Volume
100
Issue
2
Identifiers
DOI: 10.1016/s0166-6851(99)00047-x
Keywords
  • Trypanosoma Cruzi
  • Telomere Organization
  • Surface Glycoproteins
Disciplines
  • Biology

Abstract

Abstract We present here a characterization of the telomeric and subtelomeric regions of Trypanosoma cruzi chromosomes, using three types of recombinants: cosmids from a genomic library, clones obtained by a vector–adaptor protocol, and a recombinant fragment cloned by a Bal31 trimming protocol. The last nine nucleotides of the T. cruzi overhang are 5′-GGGTTAGGG-3′, and there are from 9 to 50 copies of the hexameric repeat 5′-TTAGGG-3′, followed by a 189-bp junction sequence common to all recombinants. The subtelomeric region is made of sequences associated with the gp85 /sialidase gene family, and/or sequences derived from SIRE, a retrotransposon-like sequence, and also the retrotransposon L1Tc. We discuss the possible implications of this genome organization.

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