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Structure and conformation of a novel genetically engineered polysaccharide P2

Authors
Journal
Carbohydrate Research
0008-6215
Publisher
Elsevier
Publication Date
Volume
330
Issue
3
Identifiers
DOI: 10.1016/s0008-6215(00)00293-7
Keywords
  • Polysaccharide
  • Acetobacter Xylinum
  • Acepgene
  • Nmr
  • Methylation

Abstract

Abstract A new exocellular polysaccharide (P2) has been produced by the manipulation of a glycosyl transferase gene ( aceP) involved in the biosynthesis of the polysaccharide acetan by the bacterium Acetobacter xylinum strain CKE5. The P2 polysaccharide has been studied by methylation analysis, reductive cleavage, and 1H and 13C NMR spectroscopy. The data are consistent with the structure predicted when the aceP gene is deactivated: The effect of cooling on proton NMR line width indicates a coil–helix transition in P2 at about 70 °C.

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