Affordable Access

Publisher Website

Magnetic–fluorescent Langmuir–Blodgett films of fluorophore-labeled ferritin nanoparticles

Authors
Journal
Solid State Sciences
1293-2558
Publisher
Elsevier
Publication Date
Volume
11
Issue
4
Identifiers
DOI: 10.1016/j.solidstatesciences.2008.04.026
Keywords
  • Ferritin Nanoparticles
  • Langmuir–Blodgett Films
  • Magnetic Properties
  • Fluorescent Properties
Disciplines
  • Biology
  • Chemistry
  • Physics

Abstract

Abstract We have covalently coupled fluorophore 4-(2-hydroxyethoxy)-7-nitro-2,1,3-benzoxadiazole (NBD) to the external ferritin shell through lysine residues. An increase in the luminescence quantum yield of the fluorescent ferritin particles and a blue shift in its emission peak compared to individual fluorophore were observed. The study of the particles by transmission electron microscopy showed that the native iron core ferritin is intact and that no degradation occurs during chemical functionalization of the protein shell. The NBD-labeled ferritin particles are water soluble, which allowed their controlled deposition by the Langmuir–Blodgett (LB) technique. Superparamagnetic and fluorescent properties of the particles are preserved within the LB film.

There are no comments yet on this publication. Be the first to share your thoughts.