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Colorimetric assay of urinary 5-hydroxy-3-indoleacetic acid

Authors
Journal
Clinical Biochemistry
0009-9120
Publisher
Elsevier
Publication Date
Volume
15
Issue
2
Identifiers
DOI: 10.1016/s0009-9120(82)96125-2

Abstract

We describe a method for 5-hydroxy-3-indoleacetic acid in urine based on its reaction with nitrosonaphthol in the presence of mercaptoethanolamine, an odorless compound. Mercaptoethanolamine shifts the color maximum from 540 nm to 640 nm, intensifies the color and discharges the color of interfering substances. The method is rapid and free from interferences.

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