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1H-NMR analysis of the sugar structures of glycoproteins as their pyridylamino derivatives.

Authors
  • Koyama, S
  • Daiyasu, H
  • Hase, S
  • Kobayashi, Y
  • Kyogoku, Y
  • Ikenaka, T
Type
Published Article
Journal
FEBS Letters
Publisher
Wiley (John Wiley & Sons)
Publication Date
Dec 15, 1986
Volume
209
Issue
2
Pages
265–268
Identifiers
PMID: 3792548
Source
Medline
License
Unknown

Abstract

The 1H-NMR spectra of a series of pyridylamino (PA-) derivatives of oligosaccharides were obtained and compared with those of the corresponding asparagine-linked sugar chains in order to elucidate the effect of the PA-group on the chemical shifts of structural-reporter signals. The effects were found to be localized within the two residues from the end group. Thus, the data for asparagine-linked chains in the literature are applicable to PA-derivatives, so the combination of pyridylamination and NMR measurements greatly reduces the time required for structure analysis of sugar chains of glycoproteins, because the isolation and purification of the chains as PA-derivatives are easy and efficient.

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