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THE EFFECT OF GLUTARALDEHYDE ON CATALASE : Biochemical and Cytochemical Studies with Beef Liver Catalase and Rat Liver Peroxisomes

Authors
Journal
The Journal of Cell Biology
0021-9525
Publisher
The Rockefeller University Press
Publication Date
Keywords
  • Brief Notes
  • Article
Disciplines
  • Biology
  • Chemistry

Abstract

THE EFFECT OF GLUTARALDEHYDE ON CATALASE Biochemical and Cytochemical Studies with Beef Liver Catalase and Rat Liver Peroxisomes VOLIKER HERZOG and H. DARIUSH FAHIMI . From the Harvard Pathology Unit, Mallory Institute of Pathology, Boston City Hospital, Boston, Massachusetts 0118 and the Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115 INTRODUCTION Peroxidatic activity of catalase (E .C .1 .11 .1 .6) was discovered by Keilin and Hartree (1), and it has been firmly established that, catalase oxidizes various substrates such as ethanol, methanol, nitrite, and formate (2-4) . Crystalline beef liver catalase, used as an enzyme tracer (5), and the peroxisomal catalase (6, 7) have been visualized cytochemically by a modification of the 3,3'- diaminobenzidine tetrahydrochloride (DAB)' tech- nique of Graham and Karnovsky (8), and it has been suggested that the peroxidatic activity of catalase is responsible for this reaction (9) . The 'Abbreviation used in this paper : DAB, 3,3'-diamino- benzidine tetrahydrochloride . staining of peroxisomes with DAB,, however, is seen only in aldehyde-fixed tissues (10, 11) . In contrast, Schejter and Bar-Eli have shown that treatment of crystalline catalase with glutaralde- hyde causes a marked inhibition of both the peroxidatic and the catalatic activities of the enzyme (12) . In this study, we have investigated the effect of fixation with glutaraldehyde on the catalatic and peroxidatic activity of crystalline beef liver catalase, using a variety of oxidizable substrates, including the DAB (13, 14) . Furthermore, we have studied the reaction of freshly isolated rat liver peroxisomes with DAB before and after the fixation with glutaraldehyde . Our observations THE JOURNAL OF CELL BIOLOGY • VOLUME 60, 1974 • pages 303-311 303 indicate that glutaraldehyde inhibits the catalatic activity of catalase while apparently enhancing the oxidation of several hydr

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