Abstract Different strains isolated from molds grown in sesamum were investigated for their lipolytic activity. It is found that P. chrysogenum S 1 shows appreciable lipolytic activity. The optimum pH for the mold lipase varies from a pH of 6.2 to 6.8 depending upon the nature of the mold lipase. The strains grown in sesamum cake medium containing 10% sesamum oil show an appreciable amount of lipolytic activity. The synthesis of n-butyl oleate can be effected using these mold lipases.