Affordable Access

Publisher Website

Analysis of in vitro and in vivo products of the TMV 30kDa open reading frame using antisera raised against a synthetic peptide

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
164
Issue
2
Identifiers
DOI: 10.1016/0014-5793(83)80316-0
Keywords
  • Antipeptide Antiserum
  • Solid Phase Peptide Synthesis
  • Tobacco Mosaic Virus
  • In Vitro Translation
Disciplines
  • Biology

Abstract

Abstract The peptide Tyr-Ser-Glu-Ala-Thr-Val-Ala-Glu-Ser-ASP-Ser-Phe (the predicted C-terminal 11 amino acids of the TMV 30kDa open reading frame plus an additional N-terminal Tyr residue) was synthesized by solid phase methods and used to raise antisera in rabbits. These antisera precipitated 4 major proteins (p30, p28, p19 and 18.5kDa) from in vitro translation products of TMV short rod RNA , but only one, of apparent M r = 30500, FROM TMV-infected tobacco protoplasts. This protein was made between 8 and 16 h post infection, and had [ 35S]Met-labelled tryptic peptides identical to those of in vitro synthesized p30.

There are no comments yet on this publication. Be the first to share your thoughts.