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Analytical techniques for cell fractions:XI. Rotor B-XXIII — A zonal centrifuge rotor for center or edge unloading

Authors
Publisher
Elsevier Inc.
Publication Date
Volume
26
Issue
3
Identifiers
DOI: 10.1016/0003-2697(68)90202-9

Abstract

Abstract A new zonal centrifuge rotor — the B-XXIII rotor — has been constructed that has tapered edge surfaces allowing a density gradient to be recovered either from the rotor edge or the rotor center. Experiments with a test sample containing bovine serum albumin and ragweed pollen grains showed only a small decrement in resolution when the rotor was unloaded from the edge instead of the center as is usually done. The removal of the outer portions of a gradient and the particles which it contains, and the reinsertion of a fresh gradient allow separation based on rate and on isopycnic banding density to be made sequentially in one rotor.

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