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Comparative Analysis ofIgf-2/H19Imprinted Domain: Identification of a Highly Conserved Intergenic DNase I Hypersensitive Region

Authors
Journal
Genomics
0888-7543
Publisher
Elsevier
Publication Date
Volume
24
Issue
1
Identifiers
DOI: 10.1006/geno.1994.1574
Disciplines
  • Biology

Abstract

Abstract The closely linked, reciprocally imprinted mouse genes, insulin-like growth factor II ( Igf-2) and H19, provide a significant paradigm for studies of the mechanism of parental imprinting. Most studies have focused on regions within and proximate to the genes, but an analysis of the whole region is essential to unravel how expression of these genes is controlled. A comparative long-range analysis of a 130-kb genomic region containing both genes was therefore carried out using material from normal and chromosome 7 maternal uniparental disomic embryos. We examined DNA methylation, chromatin structure assessed by hypersensitivity to DNase I, and regions that show strong conservation among mammalian species. The critical boundaries of differential DNA methylation and DNase I hypersensitivity are apparently confined within and proximate to the Igf-2 and H19 genes. However, we have identified a novel intergenic region that is conserved in mammals, GC-rich, and unmethylated in embryos and contains major DNase I hypersensitive sites.

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