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Leptospira Detection in Cats in Spain by Serology and Molecular Techniques.

Authors
  • Murillo, Andrea1
  • Cuenca, Rafaela1
  • Serrano, Emmanuel2
  • Marga, Goris3
  • Ahmed, Ahmed3
  • Cervantes, Salvador4
  • Caparrós, Cristina4
  • Vieitez, Verónica5
  • Ladina, Andrea5
  • Pastor, Josep1
  • 1 Department de Medicina i Cirurgia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, Barcelona (UAB), CP 08193 Bellaterra, Espana.
  • 2 Wildlife Ecology & Health group (WE&H), Servei d'Ecopatologia de Fauna Salvatge (SEFaS), Universitat Autònoma de Barcelona (UAB), CP 08193 Bellaterra, Espana.
  • 3 OIE and National Collaborating Centre for Reference and Research on Leptospirosis (NRL), Amsterdam UMC, University of Amsterdam, Medical Microbiology, Meibergdreef 39, 1105 AZ Amsterdam, The Netherlands. , (Netherlands)
  • 4 Clínica Felina Barcelona, CP 08015 Barcelona, Espana.
  • 5 Facultad de Veterinaria, Universidad de Extremadura, CP 10003 Cáceres, Espana.
Type
Published Article
Journal
International Journal of Environmental Research and Public Health
Publisher
MDPI AG
Publication Date
Mar 02, 2020
Volume
17
Issue
5
Identifiers
DOI: 10.3390/ijerph17051600
PMID: 32121670
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Leptospirosis is the most neglected widespread zoonosis worldwide. In Spain, leptospirosis reports in people and animals have increased lately. Cats can become infected with Leptospira, as well as be chronic carriers. The aim of this study was to determine serological antibody prevalence against Leptospira sp., blood DNA, and shedding of DNA from pathogenic Leptospira species in the urine of cats in Spain. Microagglutination tests (MAT) and blood and urine TaqMan real-time polymerase chain reaction (PCR) were performed. Leptospira antibodies were detected in 10/244 cats; with 4.1% positive results (95% confidence interval (CI): 2.1-7.18%). Titers ranged from 1:20 to 1:320 (serovars Ballum; Bataviae; Bratislava; Cynopteri; Grippotyphosa Mandemakers; Grippotyphosa Moskva; Pomona; and Proechimys). The most common serovar was Cynopteri. Blood samples from 1/89 cats amplified for Leptospira DNA (1.12%; 95% CI: 0.05-5.41%). Urine samples from 4/232 cats amplified for Leptospira DNA (1.72%; 95% CI: 0.55-4.10%). In conclusion free-roaming cats in Spain can shed pathogenic Leptospira DNA in their urine and may be a source of human infection. Serovars not previously described in cats in Spain were detected; suggesting the presence of at least 4 different species of pathogenic leptospires in the country (L. borgpetersenii; L. interrogans; L. kirschneri; and L. noguchii).

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