Affordable Access

Anchorage-dependent mammalian cell culture

McGill University
Publication Date
  • Engineering
  • Chemical.
  • Engineering


Genetically engineered anchorage-dependent human embryonic kidney (293) cells were cultured at 37$ sp circ$C on 1 mm thick sheets of a fibrous polymeric matrix having an average fibre diameter of 10.2 $ mu$m and a void fraction of 0.81 using Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 2.5 mM glutamine. Immobilization efficiencies above 70% were observed when cells were added to 100 mL spinner flasks (operating at 60 rpm) containing 70 mL of medium and two 1 x 1 cm squares of matrix (total gross area of 2 cm$ sp2$) fastened to the base of the stirrer shaft. Loadings in excess of 2.4 $ times 10 sp7$ cells per cm$ sp2$ of matrix were measured after 2 h.

There are no comments yet on this publication. Be the first to share your thoughts.