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Identification of a Protein That Recognizes a Distal Negative Regulatory Element within the Mouse Mammary Tumor Virus Long Terminal Repeat

Authors
Journal
Virology
0042-6822
Publisher
Elsevier
Publication Date
Volume
264
Issue
1
Identifiers
DOI: 10.1006/viro.1999.9969
Disciplines
  • Biology

Abstract

Abstract The mouse mammary tumor virus (MMTV) long terminal repeat contains a distal negative regulatory element (dNRE) that selectively represses activity of the proviral promoter in the absence of steroid hormone receptor-mediated activation. A protein, termed MMTV NRE-binding protein 1 (MNBP-1), that recognizes long terminal repeat sequences between −433 and −418 was identified by gel electrophoresis mobility shift assays and methylation interference footprinting in nuclear extracts of HeLa and Ltk − cells. Mutations within the defined binding site affect dNRE-mediated promoter repression in vivo. MNBP-1 has an apparent molecular mass of approximately 100 kDa as determined by gel filtration chromatography.

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