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Nuclease sensitivity of a maize HRGP gene in chromatin and in naked DNA

Authors
Journal
Plant Science
0168-9452
Publisher
Elsevier
Publication Date
Volume
78
Issue
2
Identifiers
DOI: 10.1016/0168-9452(91)90202-j
Keywords
  • Chromatin
  • Hrgp
  • Maiz
  • Nuclease
  • S1-Nuclease
Disciplines
  • Biology

Abstract

Abstract The sensitivity to different nucleases of a gene coding for a cell wall hydroxyproline-rich glycoprotein in Zea mays has been studied. Hypersensitive sites are found in the proximal 5′ flanking region of the gene. The main hypersensitive site around position −200, corresponds to a region that also shows a peculiar hypersensitivity to S1 degradation in naked negatively supercoiled DNA. The region where most of the changes in chromatin structure occurs is concentrated in approximately one nucleosome indicating that sequences for transcriptional control may be tightly clustered in plants.

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