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Protective immunity against Mycobacterium tuberculosis induced by dendritic cells pulsed with both CD8(+)- and CD4(+)-T-cell epitopes from antigen 85A.

Authors
Journal
Infection and Immunity
0019-9567
Publisher
American Society for Microbiology
Publication Date
Volume
70
Issue
3
Identifiers
DOI: 10.1128/iai.70.3.1623-1626.2002
Keywords
  • Acyltransferases
  • Amino Acid Sequence
  • Animals
  • Antigen Presentation
  • Antigens
  • Bacterial
  • Bcg Vaccine
  • Cd4-Positive T-Lymphocytes
  • Cd8-Positive T-Lymphocytes
  • Dendritic Cells
  • Epitopes
  • Female
  • Mice
  • Mice
  • Inbred Balb C
  • Molecular Sequence Data
  • Tuberculosis
  • Vaccination

Abstract

Immunization with DNA followed by modified vaccinia virus Ankara strain, both expressing the antigen 85A, induced both CD4(+)- and CD8(+)-T-cell responses in BALB/c mice. Following challenge with Mycobacterium tuberculosis, this prime-boost regimen produced protection equivalent to that conferred by Mycobacterium bovis BCG. Following immunization with dendritic cells pulsed with an antigen 85A CD4(+)- or CD8(+)-restricted epitope, alone or in combination, copresentation of both epitopes on the same dendritic cell was required for protection, demonstrating that induced CD8(+) T cells can play a protective role against tuberculosis.

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