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Structural implication of the induced immune response byBacillus thuringiensisCry proteins: role of the N-terminal region

Authors
Journal
Molecular Immunology
0161-5890
Publisher
Elsevier
Publication Date
Volume
41
Issue
12
Identifiers
DOI: 10.1016/j.molimm.2004.06.026
Keywords
  • Cry Proteins
  • Bacillus Thuringiensis
  • Immunogenicity
  • N-Terminal Region
Disciplines
  • Biology

Abstract

Abstract The potential role of the regions (carboxil and amino) of the Cry proteins in the ability of these proteins to elicit strong immune responses was investigated. Intraperitoneal immunization of mice with the homologous Cry1A protoxins (130–133 kDa), with the long C-terminal half gave rise mostly to similar, strong serum and mucosal IgG and IgM antibody response but a lower induction of these Ab by intranasal route. Remarkably, Cry3A protoxin, devoid of C-terminal half was able to induce a significant mucosal IgG, and IgM Ab as well as Cry1A protoxins, suggesting us that immunogenic abilities are not restricted to C-terminal half but N-terminal half itself could be involved. In fact, this assumption was strengthen by the strong immunogenic abilities of the Cry1A toxins, specially IgG and IgA Ab induced by both routes in different mucosal sites. These data indicate that immunogenic abilities of the Bt Cry proteins reside and depends of the N-terminal half.

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