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Biocleavable Polycationic Micelles as Highly Efficient Gene Delivery Vectors

Authors
Journal
Nanoscale Research Letters
1931-7573
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Volume
5
Issue
11
Identifiers
DOI: 10.1007/s11671-010-9716-9
Keywords
  • Nano Express
  • Material Science
  • Molecular Medicine
  • Engineering
  • General
  • Chemistry/Food Science
  • General
  • Physics
  • General
  • Materials Science
  • General
  • Nanotechnology

Abstract

An amphiphilic disulfide-containing polyamidoamine was synthesized by Michael-type polyaddition reaction of piperazine to equimolar N, N′-bis(acryloyl)cystamine with 90% yield. The polycationic micelles (198 nm, 32.5 mV), prepared from the amphiphilic polyamidoamine by dialysis method, can condense foreign plasmid DNA to form nanosized polycationic micelles/DNA polyelectrolyte complexes with positive charges, which transfected 293T cells with high efficiency. Under optimized conditions, the transfection efficiencies of polycationic micelles/DNA complexes are comparable to, or even higher than that of commercially available branched PEI (Mw 25 kDa).

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