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Requirement of successive protein syntheses for the progress of meiosis inBlepharisma

Authors
Journal
Experimental Cell Research
0014-4827
Publisher
Elsevier
Publication Date
Volume
145
Issue
1
Identifiers
DOI: 10.1016/s0014-4827(83)80013-5
Disciplines
  • Biology

Abstract

Summary Germ nuclei of Blepharisma japonicum begin meiosis within a few hours when cells of complementary mating types conjugate. We synchronized the onset of conjugation and treated cells in different stages of meiosis with 10 μg/ml cycloheximide which strongly inhibits protein synthesis in this ciliate. Cycloheximide arrested meiosis at six stages: I, between pairing of cells and swelling of germ nuclei; II, leptotene; III, zygotene; IV, pachytene; XI, interkinesis; XII, prometaphase II. Five of these arrests were rersible. Puromycin (250–500 μg/ml) also inhibited the progress of meiosis, though to lesser extents. We propose that the progession of meiosis of B. japonicum requires at least six proteins which are synthesized sequentially during meiosis.

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