Publisher Summary This chapter discusses two specific cell lines—PC12 and H146—as useful model systems for studying arterial and airway chemoreception, respectively. Both cell lines respond to acute hypoxia in a manner that compares well with their native counterparts, and so their usefulness in studying the cellular basis of such O2 sensing is well founded. These two cell lines, being readily amenable to molecular intervention, may prove more useful in studying the mechanisms underlying adaptation to chronic hypoxia. PC12 cell culture and amperometry and the PC12 cell line as a system for studying remodeling of cell function by chronic hypoxia are discussed in the chapter. The H146 cell line is presented as a potential system for studying remodeling by chronic hypoxia. For H146 cells, the limits of their use as models of native neuroepithelial body (NEB) cells are yet to be firmly established as no cell physiological data are currently available for human NEBs. Generating cells without functional mitochondria, reverse transcription–polymerase chain reaction (RT–PCR) screening for mRNA encoding human K2+ channels, and the loss of function employing antisense oligodeoxynucleotides are described in the chapter.