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PC12 cells transfected with a C-terminal fragment of the amyloid precursor protein (APP C-100), exhibit enhanced sensitivity to the calcium ionophore A23187, and diminished sensitivity to hydrogen peroxide1 Published on the World Wide Web on 4 August 1999.1

Authors
Journal
Molecular Brain Research
0169-328X
Publisher
Elsevier
Publication Date
Volume
72
Issue
1
Identifiers
DOI: 10.1016/s0169-328x(99)00228-4
Keywords
  • Alzheimer'S Disease
  • Calcium
  • Hydrogen Peroxide
  • Pc12
  • A23187
Disciplines
  • Biology
  • Medicine

Abstract

Abstract Extracellular neuritic plaques are a hallmark of Alzheimer's disease. The core protein of plaques is Aβ, a 39–43 amino acid peptide derived from the amyloid precursor protein (APP). APP C-100 is a C-terminal fragment of APP, 100 amino acids long, whose sequence includes Aβ. To determine whether APP C-100 expression alters cellular vulnerability to calcium and H 2O 2, rat PC12 cells were modified to overexpress APP C-100. Cellular survival (as measured in the MTT assay) was determined as a function of concentration for the calcium ionophore, A23187, and for H 2O 2 in APP C-100 transfectants and vector-transfected controls. APP C-100 expression significantly increased cellular vulnerability to A23187, and decreased vulnerability to H 2O 2.

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