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Acetylation of H2AZ Lys 14 is associated with genome-wide gene activity in yeast.

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Acetylation regulates deposition of Htz1 at the PHO5 promoter. Millar C B et al. Genes Dev. 2006;20:711-722 Copyright © 2006, Cold Spring Harbor Laboratory Press Acetylation regulates deposition of Htz1 at the PHO5 promoter. Levels of HA-Htz1, H3, and Htz1-K14 acetylation at the PHO5 promoter were determined by ChIP. Quantitations of enrichments at PHO5 relative to TelVIR are plotted. The schematic diagram (top) of the PHO5 promoter shows promoter nucleosomes as open circles and the 5′ ORF nucleosome as a filled circle, with UASp1 (black box), UASp2 (gray box), and the TATA box also shown. The positions of the primer sets used for PCR amplification are indicated by thick black lines above nucleosomes −1 and −2. An asterisk indicates the primer set for which the data are shown, although both primer sets gave identical results. (A) HA-Htz1 binding in cells that have wild-type (WT; black line) or mutated (4K-R; gray line) Htz1. At 0 min the cells were shifted from high- to low-phosphate media. (B) H3 binding in the Htz1-WT (black line) or Htz1-4K-R (gray line) strains. The cells were shifted from high- to low-phosphate media at 0 min. (C) HA-Htz1 binding in cells that have wild-type (WT; black line) or 4K-R (gray line) Htz1. At −60 min the cells were growing in low-phosphate medium; at 0 min phosphate was added. (D) H3 binding in wild-type (WT; black line) or 4K-R (gray line) strains. At −60 min the cells were growing in low-phosphate medium; at 0 min phosphate was added. (E) HA-Htz1 binding in cells that have wild-type (WT; black line) or 4K-R (gray line) Htz1. At −60 min the cells were growing in low-phosphate medium; at 0 min phosphate was added and samples were taken at 1, 5, 10, 30, and 60 min after phosphate addition. (F) Htz1-K14 acetylation at the PHO5 promoter under repressed (black bars) or active (gray bars) conditions. Raw data are plotted to the left; the values to the right have been normalized to bulk Htz1 levels to account for nucleosome loss during gene activity.

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