Affordable Access

Publisher Website

On the measurement of blocking antibody:Blocking of antigenic histamine release compared to maximal antigen binding by antibodies in serum

Authors
Journal
Journal of Allergy and Clinical Immunology
0091-6749
Publisher
Elsevier
Publication Date
Volume
49
Issue
1
Identifiers
DOI: 10.1016/0091-6749(72)90123-6

Abstract

Abstract A distinction is made between maximum antigen-binding capacity of serum and the degree to which antibodies in serum responsible for such binding may actually block interaction between antigen and sensitized cells. Measurement of histamine release from leukocytes by antigen previously incubated with serum was compared with release by antigen added simultaneously with serum. Antigenic release of histamine was much less blocked when cells, antigen, and serum antibody were brought together simultaneously. Under such circumstances, measurement of the in vitro blocking effect of antibodies on the functional action of antigen in histamine release yielded results which may be found more relevant to clinical manifestations than maximal antigen-binding capacity of serum.

There are no comments yet on this publication. Be the first to share your thoughts.