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GABA-like activity in in vitro slices of the rat visual cortex: immunocytochemistry and electrophysiology

Authors
Journal
Brain Research
0006-8993
Publisher
Elsevier
Publication Date
Volume
494
Issue
2
Identifiers
DOI: 10.1016/0006-8993(89)90613-6
Keywords
  • Rat Visual Cortex
  • Cortical Slice
  • γ-Aminobutyric Acid-Like Immunocytochemistry
  • Intracellular Recording
  • Inhibitory Synaptic Potential

Abstract

Abstract Using antisera raised against γ-aminobutyric acid (GABA), GABA-like immunoreactivity was determined in in vitro slices fixed after various incubation times. Synaptic efficacy of GABAergic innervation was concurrently assessed in adjacent slices by intracellular recordings. We show that GABA-like immunoreactivity dramatically decreases already after one hour of incubation and remains low throughout the following hours. Nevertheless robust GABA-mediated synaptic potentials are recordable for more than 8 h.

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