Affordable Access

Publisher Website

Enzyme-linked immunosorbent assay for human pulmonary surfactant protein D

Authors
Journal
Journal of Immunological Methods
0022-1759
Publisher
Elsevier
Publication Date
Volume
173
Issue
2
Identifiers
DOI: 10.1016/0022-1759(94)90295-x
Keywords
  • Surfactant Protein D
  • Monoclonal Antibody
  • Sandwich Elisa
Disciplines
  • Biology
  • Medicine

Abstract

Abstract We developed a simple and sensitive enzyme-linked immunosorbent assay (ELISA) for human pulmonary surfactant protein D (SP-D). Human SP-D was purified from bronchoalveolar lavage fluids of patients with pulmonary alveolar proteinosis. Nine monoclonal antibodies (MAbs) were established from BALB/c mice immunized of patients with pulmonary alveolar proteinosis or in amniotic fluids from healthy normal pregnancies. The ELISA is based on a sandwich method using two MAbs, 6B2 and 7C6. Cross-reactivity to human SP-A or rat SP-D was evaluated as below 0.6%. The recovery of different concentrations of SP-D ranged from 94.4% to 111.2%, and serial dilutions of amniotic fluids showed good linearity. SP-D concentrations in 21 amniotic fluids from normal pregnancies were measured by the ELISA. The mean concentrations in amniotic fluids pregnancies in the third trimester was significantly higher than that from earlier stages of gestation ( p < 0.001), indicating that this ELISA may be applicable for prediction of fetal lung maturity.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Enzyme-linked immunosorbent assay for human pulmon...

on Journal of Immunological Metho... Aug 01, 1994

Enzyme-linked immunosorbent assay using F(ab')2 fr...

on Clinica Chimica Acta Oct 31, 1997

Development of an enzyme-linked immunosorbent assa...

on Experimental lung research October 2010
More articles like this..