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The interactions between nucleic acids and polyamines:II. Protonation constants and 13C-NMR chemical shift assignments of spermidine, spermine, and homologs

Authors
Journal
Biophysical Chemistry
0301-4622
Publisher
Elsevier
Publication Date
Volume
17
Issue
1
Identifiers
DOI: 10.1016/0301-4622(83)87015-x
Keywords
  • Polyamine
  • Protonation Constant
  • 13Nmr
  • Spermine: Spermidme: Microprotonation Constant

Abstract

Abstract Macroscopic protonation constants have been determined by pulentiometric titration for spermidine, spermine and for the four pulyamines. 3,5-Spd, 4,4-Spd, 4,3,4-Spm and 4,4,4-Spm. which are homologs of spermidine and spermine. A method for calculation of microscopic protonation constants of polyamines based on data for mono- and diamines gives results for spermidine that agree well with the experimental macroscopic protonation constants and the protonation sequence of Kimberly and Goldstein. 13C-NMR spectra of spermidine, spermine and six homologs have been obtained and used to assign specific resonances, correcting some ambiguity in the assignments for spermidine and some errors in the assignments for spermine.

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