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Immunoglobulin heavy-chain joining genes in the rat: comparison with mouse and human

Authors
Journal
Gene
0378-1119
Publisher
Elsevier
Publication Date
Volume
102
Issue
2
Identifiers
DOI: 10.1016/0378-1119(91)90087-r
Keywords
  • Recombinant Dna
  • Jgene Segments
  • Pseudogene
  • Nucleotide Sequence
  • Louvain Rat
  • Polymerase Chain Reaction

Abstract

Abstract We have cloned by the polymerase chain reaction a 2.1-kb fragment carrying heavy-chain joining ( J H) gene segments and a part of the J H- C μ intron of the rat. Sequencing showed that the rat genome has four functional J H segments and that only a slight divergence has occurred after the separation of rat and mouse. A systematic sequence comparison between the two species and human revealed an additional J H pseudogene in rat and mouse 5′ of J H1 which has not been described so far. The implications in evolutionary terms are discussed. In addition, we give an assessment of the misincorporation rate of the Taq polymerase.

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