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Preparation and adsorption of bovine serum albumin-imprinted polyacrylamide hydrogel membrane grafted on non-woven polypropylene

DOI: 10.1016/j.talanta.2014.01.010
  • Protein Molecular Imprinting
  • Polypropylene Non-Woven
  • Grafting
  • Polyacrylamide Hydrogel
  • Membrane
  • Adsorption
  • Biology


Abstract Bovine serum albumin (BSA) imprinted polypropylene (PP) fiber-grafted polyacrylamide (PAM) hydrogel membrane (PP-g-PAM MIP) was prepared using non-woven PP fiber as matrix, BSA as template molecule, and acrylamide (AM) as functional monomer via UV radiation-reduced polymerization in an aqueous phase. SEM, FT-IR, DSC and TG were used to characterize the PP grafted PAM hydrogel. Influence factors on the adsorption capacity of PP-g-PAM MIP were investigated, such as monomer concentration, cross-linker concentration, template molecule amount and pH values in BSA solution. The adsorption and recognition properties of PP-g-PAM MIP were evaluated and the results showed that the PP-g-PAM MIP exhibited an obvious improvement in terms of adsorption capacity for BSA as compared with non-imprinted ones. PP-g-PAM MIPs could recognize the template protein using Lys, Ova, BHb, and Glo as control proteins, and the selectivity factor (β) was above 2.0. The imprinting efficiency of PP-g-PAM MIP tended to be stable after three cycles and maintained 76% of the initial value of the imprinting efficiency even after five repetitions, which was more excellent than that of PAM microsphere. The PP-g-PAM MIP is low cost and easy to be prepared, which would show its potential applications in the fields of extracting and testing required proteins from cells or particulate samples.

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