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Adsorption chromatographic separations on buffered silica gel

Authors
Journal
Journal of Chromatography A
0021-9673
Publisher
Elsevier
Publication Date
Volume
334
Identifiers
DOI: 10.1016/s0021-9673(00)80264-2

Abstract

Abstract HPLC separations of polar solutes, by adsorption chromatography on silica gel, is often hindered by tailing of the bands. By treating the silica gel with a buffer salt, insoluble in the mobile phase, an environment is created on the surface of the adsorbent that permits tail-free elution of very polar compounds. The influence of the nature of the buffer salt and of its concentration and pH has been demonstrated. Buffered silica gel is not only an alternative to reversed-phase chromatography but may also be superior to it for the separation of isomers or for semi-preparative separations.

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