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Methylation of lysine residues of histone fractions in synchronized mommalian cells

Authors
Journal
Archives of Biochemistry and Biophysics
0003-9861
Publisher
Elsevier
Publication Date
Volume
143
Issue
1
Identifiers
DOI: 10.1016/0003-9861(71)90180-9

Abstract

Abstract Synchronized cultures of mammalian cells were labeled with 14C-methyl methionine. Labeled methionine methyl groups were incorporated into certain histone fractions, forming methyl lysine. Incorporation of labeled methyl group into histone fractions as 14C-methyl lysine was followed through the cell cycle from late G 1 into early M. The 14C-methyl lysine contents of fractions F2a and F3 began to rise in S and reached maxima after termination of DNA and histone synthesis, coincident with the beginning of mitosis, and began to fall by mid- M. The 14C-methyl lysine content of fraction F2b rose to a maximum early in S, coincident with initiation of DNA synthesis, and rapidly decreased to its original unmethylated level by late S. Fraction F1 remained unmethylated during the period G 1- M. Evidence is presented to demonstrate differential methylation of histone fractions and to substantiate differential temporal coupling of the methylation of specific histone fractions with histone and DNA biosynthesis.

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