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A variation of the tissue print technique for studying isolated spinal cord cells in situ

Authors
Journal
Neuroscience Letters
0304-3940
Publisher
Elsevier
Publication Date
Volume
223
Issue
2
Identifiers
DOI: 10.1016/s0304-3940(97)13423-1
Keywords
  • Tissue Print
  • Cell Isolation
  • Method
  • Whole Cell
  • Patch Clamp
Disciplines
  • Biology

Abstract

Abstract We present a novel variation of the tissue print technique which yields isolated spinal cells that retain their original topographical organization in situ. A light papain digestion combined with a low-melting-point agarose as the adhesive substrate is used to `print' a monolayer of cells from spinal cord slices. Printed cells are viable for studies using electrophysiological approaches. This technique provides a means to bridge the gap between properties of isolated cells and their topographical identity in spinal cord.

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