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Uptake of sensitizer by electroporated yeast cells

Authors
Journal
Bioelectrochemistry and Bioenergetics
0302-4598
Publisher
Elsevier
Publication Date
Volume
47
Issue
1
Identifiers
DOI: 10.1016/s0302-4598(98)00160-3
Keywords
  • Saccharomyces Cerevisiae
  • Thiopyronine
  • Electroporation

Abstract

Abstract The photodynamic effect on yeast ( Saccharomyces cerevisiae) cell viability has been enhanced by electroporation treatment. Exponentially decaying high-voltage pulses caused temporary permeability changes on the cell walls, by which the uptake of the sensitizer thiopyronine (TP) has been accelerated. The total killing effect after visible light irradiation, determined by Phloxine B exclusion tests, depended on the pulse strength and the TP concentration as well as the irradiation time. Under optimal condition, 0.01 mM TP and pulse strength 2.35 kV/cm, it was shown that the effect after 5 min irradiation increased to more than 10 times, e.g., 75–80% cells are dead, as much as the control sample, which was treated only by TP and irradiation but without electroporation.

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