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19-nor-DOC biosynthesis in the isolated perfused rat kidney.

Authors
  • Azar, S T
  • Melby, J C
  • Holbrook, M M
  • Wilson, T E
  • LaRaia, J
  • Lieberthal, W
Type
Published Article
Journal
The Journal of Steroid Biochemistry and Molecular Biology
Publisher
Elsevier
Publication Date
Dec 01, 1991
Volume
39
Issue
6
Pages
937–939
Identifiers
PMID: 1751393
Source
Medline
License
Unknown

Abstract

19-nor-deoxycorticosterone (19-nor-DOC) is a potent salt retaining and hypertensinogenic mineralocorticoid that is excreted in the urine. While the precursor of 19-nor-DOC, 19-oxo-DOC, is produced by the adrenal cortex, conversion to 19-nor-DOC does not occur in the adrenal gland. We have examined the hypothesis that 19-nor-DOC is synthesized from precursors in the kidney. 19-oxo-DOC was added to the perfusate of isolated rat kidney preparations (n = 5) at a concentration of 10 microM. During 1 h of perfusion following addition of 19-oxo-DOC, 71 +/- 6% of the precursor was converted to 19-oic-DOC, an immediate precursor of 19-nor-DOC, and 8.3 +/- 1.8% was converted to 19-nor-DOC. This represents the first definitive evidence that 19-nor-DOC is produced in the kidney from adrenal precursors.

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