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The potency of the fatty acid amide hydrolase inhibitor URB597 is dependent upon the assay pH

Authors
Journal
Pharmacological Research
1043-6618
Publisher
Elsevier
Publication Date
Volume
54
Issue
6
Identifiers
DOI: 10.1016/j.phrs.2006.07.006
Keywords
  • Anandamide
  • Fatty Acid Amide Hydrolase
  • Urb597
  • Ph Dependency
  • Carbamylation
Disciplines
  • Biology

Abstract

Abstract Inhibitors of the enzyme fatty acid amide hydrolase (FAAH), the principal enzyme involved in the metabolism of the endogenous cannabinoid anandamide, have potential utility in the treatment of disorders including inflammation and inflammatory pain. The carbamate compound URB597 (3′-carbamoyl-biphenyl-3-yl-cyclohexylcarbamate) potently and selectively inhibits FAAH by forming a covalent bond with a key serine residue of the enzyme. Little is known as to the pH dependency of this inhibition. Using a preincubation time of 10 min, URB597 inhibited rat brain anandamide hydrolysis with p I 50 values of 7.19 ± 0.02 and 7.75 ± 0.06 at pH 6 and 8, respectively. The inhibition was time-dependent, and second order rate constants of ∼0.15 × 10 6 M −1 min −1 (pH 6) and ∼1.2 × 10 6 M −1 min −1 (pH 8) could be estimated. In intact C6 glioma cells and using a preincubation time of 10 min, URB597 inhibited the hydrolysis of 250 nM [ 3H]AEA hydrolysis with p I 50 values of 5.58 ± 0.07 and 6.45 ± 0.07 at extracellular pH values of 6 and 8, respectively. Since tissue pH is affected by inflammation, these data would suggest that the pH selectivity of the inhibition can contribute to the potency of the compound in vivo.

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