Affordable Access

Publisher Website

Detection of Elevated Basic Fibroblast Growth Factor During Early Hours ofin VitroAngiogenesis Using a Fast ELISA Immunoassay

Biochemical and Biophysical Research Communications
Publication Date
DOI: 10.1006/bbrc.1994.2824
  • Biology


Abstract Basic FGF (bFGF) is a growth factor that is thought to play an important role in angiogenesis. Available assays that are used to detect bFGF are long and cumbersome. Here, we present a fast, easy and sensitive sandwich-type enzyme immunoassay for bFGF detection. Our method is a modification of the method described by Watanabe et al (Biochem. Biophys. Res. Commun. 1991;175, 229). Two monoclonal antibodies for antigen capture and one noncongugated polycolonal antibody for antigen detection are used instead of using three monoclonal antibodies with the congugation of one of them for detection. There is no change in the sensitivity of the assay with average detection limit of 1 pg/well. Acidic fibroblast growth factor does not interfere with the assay. Using this method, samples from conditioned media of capillary endothelial cell culture before and after angiogenesis were measured. Associated with detection of start of tube formation, basic FGF was elevated at 8 hours from angiogenic stimulation and peaked at 48 hour (4 times control), showing for the first time in an in vitro system that there is a transient increase in endogenous bFGF accompanying early steps of angiogenesis which in turn may be the trigger for new capillary formation.

There are no comments yet on this publication. Be the first to share your thoughts.


Seen <100 times

More articles like this

Detection of elevated basic fibroblast growth fact...

on Biochemical and Biophysical Re... Dec 15, 1994

Quantitation of basic fibroblast growth factor by...

on Journal of Pharmaceutical and... Jan 01, 2000

Quantitation of basic fibroblast growth factor by...

on Journal of Pharmaceutical and... Dec 15, 2000

Improved enzyme immunoassay for human basic fibrob...

on Biochemical and Biophysical Re... Jun 15, 1993
More articles like this..