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Blood pH in Vivo : II. The Effect of Acids, Salts, Dextrose, and Adrenalin *

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BLOOD pH IN VIVO II. THE EFFECT OF ACIDS, SALTS, DEXTROSE, AND ADRENALIN* CLYDE MARSHALL AND LESLIE F. NIMS Various materials injected into the blood stream of living animals alter the acidity of blood, as has been proven by many experimenters. The usual method employed to follow the changes has been to analyze samples of blood drawn at selected intervals. It has been realized that while this is a valid procedure for changes that take place slowly, it is totally inadequate when rapid changes are involved. Since it is now possible to record directly the acidity of circulating blood in living animals, it is apparent that the problems involved in rapid changes of blood acidity should be further investi- gated by this new technic. This paper reports the effect on the acidity of arterial blood of the intravenous injection of certain acids, salts, and other substances. Experimental Methods and Results Cats and dogs were anesthetized with nembutal and were given the azo dye "chlorazol fast pink" intravenously to prevent coagu- lation of the blood. A cannula, designed to hold a glass and a reference electrode, was tied into the left carotid artery. The elec- trodes were placed in position and connected with the apparatus for continuous recording. A blood pressure cannula was inserted into the right carotid artery and an injection cannula into the right femoral vein. Some of the animals were curarized and kept under artificial respiration in order to differentiate between those changes in blood acidity brought about primarily by variations in ventilation and those produced through other physiological mechanisms. Com- plete experimental details of the method employed have been given previously.' 2,3 Hydrochloric acid injected into the right femoral vein of a curarized dog produced an abrupt acid wave of the blood in the left carotid artery approximately nine seconds after the injection (Fig. IA). The blood then returned towards the alkaline side with a *From the Laboratories of Neuro-Anatomy and Phy

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