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High-pressure liquid chromatography analysis of oligo- and monoribonucleotide mixtures, with special reference to ribosomal RNA constituents

Authors
Publisher
Elsevier Inc.
Publication Date
Volume
107
Issue
2
Identifiers
DOI: 10.1016/0003-2697(80)90398-x
Keywords
  • Chromatographic And Electrophoretic Techniques

Abstract

Abstract High-pressure liquid chromatography on columns of commercial anion exchangers, with aqueous buffers as eluents, is used in two types of separation. Oligonucleotide mixtures are fractionated according to chain length at neutral pH in the presence of urea, and mononucleotides are separated according to base composition using acidic buffers. These procedures are applied to the quantitative assay of alkali-resistant oligonucleotides, and to the determination of base composition and pseudouridylic acid content, of Artemia salina ribosomal RNA. High-pressure liquid chromatography permits rapid and extremely sensitive assays not requiring a radioactive label, and therefore compares favorably with other fractionation procedures for RNA components in some specific applications.

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