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Removal of Triton X-100 from aqueous solution using amberlite XAD-2

Authors
Publisher
Elsevier Inc.
Publication Date
Volume
92
Issue
2
Identifiers
DOI: 10.1016/0003-2697(79)90683-3
Keywords
  • Chromatographic And Electrophoretic Techniques
Disciplines
  • Biology

Abstract

Abstract Amberlite XAD-2 beads adsorb the nonionic detergent Triton X-100 over a wide range of conditions with a maximum capacity of 0.475 mmol/g dry weight. After treatment of protein solutions containing Triton X-100 with XAD-2 no interference by Triton with Folin assays was observed. Adsorption of Triton X-100 was favored by a free-energy change of −835 cal/mol and by a positive entropy value. Adsorbed Triton could be completely desorbed and the XAD-2 regenerated with little loss in capacity by washing with propan-2-ol. Removal of Triton by XAD-2 was also successful in columns or bateh-wise on a pilot-plant scale.

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