Affordable Access

Publisher Website

The Large Subunit of Replication Factor C Promotes Cell Survival after DNA Damage in an LxCxE Motif– and Rb-Dependent Manner

Authors
Journal
Molecular Cell
1097-2765
Publisher
Elsevier
Publication Date
Volume
7
Issue
4
Identifiers
DOI: 10.1016/s1097-2765(01)00217-9
Disciplines
  • Biology

Abstract

Abstract Retinoblastoma (Rb) protein promotes cell survival after DNA damage. We show here that the LxCxE binding site in Rb mediates both cell survival and cell-cycle arrest after DNA damage. Replication factor C (RF-C) complex plays an important role in DNA replication. We describe a novel function of the large subunit of RF-C in promoting cell survival after DNA damage. RF-Cp145 contains an LxCxE motif, and mutation of this motif abolishes the protective effect of RF-Cp145. The inability of wild-type RF-Cp145 to promote cell survival in Rb-null cells is rescued by Rb but not by Rb mutants defective in binding LxCxE proteins. RF-C thus enhances cell survival after DNA damage in an Rb-dependent manner.

There are no comments yet on this publication. Be the first to share your thoughts.