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Rapid method for converting fungal cells into protoplasts with a high regeneration frequency

Authors
Journal
Experimental Mycology
0147-5975
Publisher
Elsevier
Publication Date
Volume
8
Issue
4
Identifiers
DOI: 10.1016/0147-5975(84)90064-1
Disciplines
  • Biology

Abstract

Abstract Protoplasts with a high regeneration capacity were obtained from mycelia of Aspergillus oryzae M-13 by a rapid procedure using a lytic enzyme system composed ofβ-glucanase and chitinase. Protoplasts were concentrated and recovered free from cell debris and lytic enzymes by low-speed centrifugation. When incubated on agar plates, the protoplasts regenerated at a rate of 99.6%.

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