Patterns of localization of coat protein-GFP fusions during spore formation. Driks A J. Bacteriol. 2004;186:4423-4426 Patterns of localization of coat protein-GFP fusions during spore formation. Spore formation proceeds from top to bottom. The cells in a row are at approximately equivalent stages. (A) YabP-GFP. Early in spore formation, immediately after the septum appears, YabP-GFP (blue) localizes to the mother cell side of the septum (top cell). Mother cell (MC) and forespore (FS) compartments are indicated. As forespore engulfment continues (next cell down), foci (blue dots) appear at the leading edge of the septum. In fully engulfed forespores, the foci are located on either side of the short axis of the forespore (next cell down). YabP-GFP is delocalized prior to mother cell lysis (bottom cell). (B) YheD-GFP. After engulfment, four foci (pink) are present, on either side of the short axis of the forespore, suggesting that there are two rings (upper cell). The sizes of the dots indicate relative levels of fluorescence. These rings become a contiguous shell (lower cell). (C) YutH-GFP. A focus (orange) is present after septum formation (top cell). This focus becomes a ring after forespore engulfment (next cell down) and then a cap at the mother cell pole (next cell down). Finally, the cap becomes a complete shell (bottom cell).