Affordable Access

Publisher Website

Quantitation of bergenin in human plasma by liquid chromatography/tandem mass spectrometry

Authors
Journal
Journal of Chromatography B
1570-0232
Publisher
Elsevier
Publication Date
Volume
877
Identifiers
DOI: 10.1016/j.jchromb.2008.11.005
Keywords
  • Bergenin
  • Lc–Ms/Ms
  • Pharmacokinetics
Disciplines
  • Pharmacology

Abstract

Abstract This paper reports the development and validation of an assay for quantitation of bergenin in human plasma using liquid chromatography/tandem mass spectrometry (LC–MS/MS). Bergenin and the internal standard (I.S.), 5-bromo-2,4(1 H,3 H)-pyrimidinedione (5-BrU), were separated by reversed phase HPLC and quantitated by MS/MS using electrospray ionization (ESI) and multiple reaction monitoring (MRM) in the negative ion mode. The most intense [M−H] − MRM transition of bergenin at m/ z 326.9 → 312.3 was used for quantitation and the transition at m/ z 188.9 → 42.2 was used to monitor 5-BrU. Stability issues with bergenin required the addition of ascorbic acid to plasma samples prior to storage and analysis within 10 days storage at −80 °C. The method was linear in the range 3–1000 ng/mL with intra- and inter-day precision of 3.94–5.96 and 1.62–8.31%, respectively, and accuracy <2.33%. The assay was successfully applied to a pharmacokinetic study in healthy volunteers after administration of a single 250 mg oral dose.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Quantitation of Armillarisin A in human plasma by...

on Journal of Pharmaceutical and... Apr 11, 2007

Rapid and sensitive liquid chromatography-tandem m...

on Journal of Chromatography B May 05, 2005

Quantitation of nitrofurantoin in human plasma by...

on Acta pharmaceutica (Zagreb, Cr... June 2013

Quantitation of the tacrine analogue octahydroamin...

on Journal of Pharmaceutical and... Jan 01, 2009
More articles like this..